28-year-old Khan has only fought once previously at this weight, which heralded a stellar performance against the experienced Luis Collazo in May.
The Bolton-born boxer will be hoping for a similar outcome against 27-year-old Alexander on Saturday - at the same venue that he beat Collazo.
In only his second fight as welterweight, Alexander won the IBF world title with a unanimous decision over Randall Bailey but subsequently lost his belt two fights later against Shawn Porter.
Both fighters will be looking to produce a knockout performance in the hoping of landing a dream fight against either Floyd Mayweather or Manny Pacquiao.

